One of the main limitations of this technique is the low number of sexed sperm produced per unit of time, and sexed sperm display a variety of damages, viz. L, Gouraud A, Holt W.
After further culture and development in vitro, the embryos are transferred to a recipient female resulting in the birth of live offspring.
For many people, development of these therapies represents the difference between their current symptoms and a disease-free future.
This efficiency is much lower in buffalo, apart from lower recovery of oocytes in this species. A, Reichenbach H. C, Mezzalira A. Multivariate correction did not remove the significance of the association of birth defects and ICSI corrected odds ratio 1.